Dr. KAUSTUBH MULAY

Dr. SANTOSH G. HONAVAR

Abstract

Objective: To evaluate the role of squash cytology in ophthalmic tumors, it’s diagnostic accuracy and statistical significance (sensitivity, specificity, positive predictive value and negative predictive value) following histological confirmation.

Materials and methods: A total of 143 ophthalmic tumors were included. Squash smears and frozen sections were prepared in each. Smears were stained with May-Grunewald-Geimsa and Pap stains while frozen sections were stained with H&E. Rest of the tissue was use to prepare FFPE tissue and used for reaching a final diagnosis. Results were compared.

Results: H&E diagnosis was taken as gold standard. By comparing results squash preparation has a sensitivity of 97.3%, specificity of 86.4%, PPV of 98.7% and NPV of 88.5%.

Conclusion: Despite it’s pitfalls and various limitations, squash cytology is a rapid and easy method with fairly high diagnostic accuracy. It can this be reliably used an intraoperative diagnostic tool in Ophthalmic tumors.

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