Dr. POOJA KHAMAR

Dr. ROHIT SHETTY

Abstract

Purpose:

To evaluate the role of rapid, multiplexed diagnostic kit, in tears of patients undergoing Refractive Surgery, Keratoconus(KC) Dry eye Disease treatment(DED) and ocular surface .

Methods:

1080 controls, 1000 KC and post-refractive surgery, 1300 DED, were measured. Tear samples from schirmer’s strips were added to the test cartridge and inserted into a laser based detection platform(ELISA Based) to determine the concentration of the analyte on the basis of a standard curve.

Results:

IL-6, MMP-9 were highest in DED and subjects with DED as a co-morbidity. Interestingly, in KC, subset of subjects had high MMP9. Interleukin1 beta and IL10 was found low in controls but high in specific patient groups, indicating use for treatment customisation. This rapid, multiplexed kit detected analytes with a 120min turn around time in the clinic.

Conclusion:

Point-of-care tear diagnostic kits can thus revolutionize screening, treatment and monitoring protocols in ocular surface conditions.

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