DR. DIVYA TRIVEDI

Dr. ROHIT SHETTY, Dr. POOJA KHAMAR, Dr. SHARON DSOUZA

Abstract

Purpose: To study expression of host response genes associated with SARS-CoV-2 attachment, entry and replication; and to develop strategies for ocular surface prophylaxis and therapy. Methods: Cadaveric eyes were dissected by layers-corneal epithelium, stroma, conjunctival epithelium, iris, lens, retina etc.,(n=5). Expression of host pro-viral(ACE2, TMPRSS2, CTSL) & antiviral(interferons) response genes were measured in these layers and also after treating human corneal epithelial cells in vitro with trehalose. Results: ACE2, TMPRSS2 are expressed in higher levels in corneal epithelium, stroma, conjunctival epithelium compared to other layers. Trehalose reduced the expression of ACE2, TMPRSS2 and induced IFN alpha. Conclusion: Ocular surface is at high risk of infection susceptibility as it has high expression of viral receptors and due aerosolised virus. Topical agents (trehalose) may render prophylaxis by reducing SAR-CoV-2 entry receptors expression and inducing anti-viral IFNs.

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